MIRI® Time-Lapse Incubator

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MIRI® Time-Lapse Incubator
MIRI® Time-Lapse Incubator Top Right

MIRI® Time-Lapse Incubator

The MIRI® Time-Lapse Incubator is a multiroom incubator with a built-in camera and microscope. Designed and manufactured in EU, the MIRI® Time-Lapse Incubator provides high quality time-lapse images of embryos developing in "real-time" without having to remove the embryos from the safety of the incubation chamber for manual microscopy. Time-lapse embryo monitoring provides detailed morphokinetic data throughout embryo development, which is not available on routine spot microscopic evaluation. This allows all important events to be observed, helping to identify healthy embryos with the highest probability of implantation, with the aim of achieving higher pregnancy rates.

Time-Lapse
Animal IVF

For Time-Lapse & Animal IVF

Features

Completely Independent Chambers

Time-Lapse Monitoring

Heated Lids

Fast Temperature and Gas Recovery

Easy External Gas and Temperature Validation

Superior Gas System

User Friendly Interface

The CultureCoin®

MIRI® TL Viewer Software and MIRI® TL Server

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Product Details Product Features Introduction Simple guide on how to operate the MIRI® TL General Specifications Ordering Information Literature & Resources

Completely Independent Chambers

MIRI® TL Multiroom IVF incubators  have 6 (TL6) or 12 (TL12) independent chambers where each chamber can be designated to one patient to avoid contamination. These chambers are equipped with precise temperature and gas regulation systems that allow to set individual temperature set-points and open any chamber without affecting the environment or time-lapses in other chambers.
Completely Independent Chambers
Time-Lapse Monitoring

Time-Lapse Monitoring

MIRI® TL Multiroom IVF incubators feature the ability to generate time-lapse videos from images that allows objective and reliable scoring with the ability to annotate the embryos for better prediction of future developmental and implantation potential. 

Heated Lids

MIRI® TL Multiroom IVF incubators’ incorporated heated lids prevent condensation build-up, help to achieve greater consistency throughout each chamber, and significantly improve temperature regulation and recovery of the system.
Heated Lid

Fast Temperature and Gas Recovery

MIRI® TL Multiroom IVF incubators have remarkable gas and temperature recovery rates, with concentration and temperature levels in the chambers returning to normal in less than 3 and 1 minute, respectively.*

*If the lid has not been open for more than 30 seconds.

Easy External Gas and Temperature Validation

MIRI® TL Multiroom IVF incubators have integrated gas sampling ports and independent PT-1000 sensors for each chamber that allow the external quality evaluation of gas levels and temperature in each chamber using own or Esco Medical provided MIRI® GA Gas and Temperature Validation Unit.

Superior Gas System

MIRI® TL Multiroom IVF incubators have a built-in gas mixer for tri-gas, that allows users to change the gas composition of the culture media without the need to order and try different pre-mixed gases. These incubators also include advanced gas regulation and VOC/HEPA filtered recirculation systems that enable precise gas level control and lowers gas consumption.
Moreover, integrated UV-sterilization of recirculating gas ensures biological safety of embryos.

User Friendly Interface

User Friendly Interface

MIRI® TL Multiroom IVF incubators include a touchscreen control panel with an intuitive menu to manage device configuration.

The CultureCoin®

It is a specifically designed culture dish that enables easier loading and culturing of up to 14 embryos in each chamber, giving the total capacity of 84 and 168 embryos to MIRI® TL6 and TL12 variants, respectively.

MIRI® TL Viewer Software and MIRI® TL Server

MIRI® TL Multiroom IVF incubators come with a dedicated Viewer software that helps users process the data generated by the incubators. It also includes a patient database for entering patient and treatment details. A built-in computer with a storage capacity of 256 GB ensures the ability to store time-lapse images in the event of a faulty connection between the incubator and the Viewer software.

MIRI TL Viewer Main Screen

Introduction

Timelapse Technology

Time-lapse Technology:
Addressing IVF Risks

Time-lapse technology is a modern system used in the practice of Clinical Embryology for the culture and imaging of embryos in vitro. In recent years, the risks associated with In Vitro Fertilization (IVF), such as multiple pregnancies and higher rates of mother and neonate mortality, have increased.

Although stricter policies on embryo transfer have reduced some of the risks involved, the occurrence of twins is still a concern. It is believed that a twin pregnancy can be avoided by opting for a single embryo transfer. However, the traditional method of selecting embryos based on their morphology is not accurate enough to allow for routine single transfers. Thus, new and more effective screening tools are needed.

By utilizing a time-lapse incubator, it is possible to continuously monitor embryos without disturbing their optimal culture conditions. This non-invasive method enables IVF practitioners to observe important information such as cleavage patterns, morphological deviations, and the dynamics of embryo development. Constant monitoring can aid in identifying embryos with higher implantation potential, allowing for unbiased selection of embryos for transfer. This technological advancement is a significant step forward in the field of IVF.
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The Silent Embryo Hypotesis

The Silent Embryo Hypothesis

Baumann et al wrote that “The Silent Embryo hypothesis proposes that viable preimplantation embryos operate at metabolite or nutrient turnover rates distributed within lower ranges than those of their less viable counterparts”. 

The MIRI® TL Multiroom IVF incubator is a multi-room incubator equipped with a built-in microscope and camera that continuously captures images of embryo development without disruption until the day of transfer. This allows for a detailed scoring of the cultured embryos, which can better predict their developmental and implantation potential. 

In conventional assessment of embryo selection in IVF, observations are often limited to pre-defined time intervals, which requires removing the embryo from the incubator. With the MIRI® TL Multiroom IVF incubator, the dynamic process of embryo development can be observed without disturbing the culture conditions. This supports the Silent Embryo Hypothesis, which suggests that embryos develop better when they are less disturbed.

Simple guide on how to operate the MIRI® TL

I. CultureCoin® Preparation

  1. Leave the dishes with the covers on for off-gassing in the aseptic environment for 12 hours (overnight).
  2. Warm the dishes on a heated stage or an incubator before filling them with media.
  3. Fill all the culture wells with approximately 25 µl of precultured equilibrated culture media in an aseptic environment.
  4. Filling of the washing wells is optional. One washing well contains approximately 23 µl of the solution. They can be used for cell manipulations, washing media or replacement media.
  5. Check under a microscope for any visible air bubbles and remove them with a stripper tip. 
  6. Fill the oil area with a confluent layer of suitable mineral or Paraffin oil to cover the culture media. The recommended amount is 6 ml. Do not overfill. 
  7. Put the cover on the dish and leave it for 4 hours of equilibration in the CO2 incubator (humidified incubator is preferable).
CultureCoin® - Embryo Culture Dish for the MIRI® TL

II. Loading of the Embryos

  1. Check for air bubbles in the culture wells after the equilibration. Remove any bubbles with a stripper tip. 
  2. Load the embryos in the culture wells. Make sure the embryos are located very precisely in the center of the culturing well and not on the side slope.
  3. Optionally fill the pH well with culture media up to the lowest step (recommended volume – 350 µl) of the pH well and cover it with an oil overlay (recommended volume – 150 µl).
  4. Once done, put the CultureCoin® in the MIRI® TL family’s multiroom IVF incubator.
  5. Select the patient, then select or deselect desired active positions and start the time-lapse when ready.

III. Embryo Annotation

  1. On the MIRI® TL Viewer's Home Screen, select “Timelapses” and look for the patient to open time-lapse videos. A revolver view with the time-lapse video of each of the 14 wells in the CultureCoin® will be displayed.
  2. Select an embryo to annotate. The embryo will be maximized at the center. The annotation system is structured around the “events” that are located on the left of the wheel.
MIRI TL 6 And viewer

IV. Selecting the most Viable Embryos

MIRI TL Viewer Main Screen
  1. The MIRI® TL Viewer software is equipped with sophisticated tools to help you evaluate and select the most viable embryo after completely annotating all embryos.
  2. The Compare Tool will let you do a side-by-side comparison of two embryos. Two embryo videos can be played independently or linked together for comparison.
  3. The “Summary” tool will allow you to compare all embryos in an informative and graphical way. It will let you compare embryo developmental timings and which one develops closely with the ideal timings.
  4. Once the selection process has been completed or a decision has been made, click the green check icon in the upper left corner. After clicking it, a drop-down list of the desired status will appear.
  5. Assign a corresponding color that indicates the decision in each well: Green for “transfer”, Red for “discard”, and Blue for “freeze”. The colored ring will appear around the well to indicate the decision made. You can also click the “Dish Map” icon to visually map the decision that has been made.

General specifications for the MIRI® TL6 and TL12 Multiroom Incubators

Specifications MIRI® TL6 MIRI® TL12
Overall Dimensions (W x D x H) 805 x 590 x 375 mm (31.7 x 23.2 x 14.8") 950 x 685 x 375 mm (37.4 x 27 x 14.8")
Weight 60 kg (132.3 lbs) 93 kg (205 lbs)
Material Mild steel / Aluminum / PET / Stainless steel Mild steel / Aluminum / PET / Stainless steel
Power Supply 115V 60Hz or 230V 50Hz 115V 60Hz or 230V 50Hz
Power Consumption 330 W 650 W
Temperature Control Range 28.7 - 41 °C 28.7 - 41.0 °C
*CO2 Gas Consumption <2 Liters per hour <2 Liters per hour
**N2 Gas Consumption <5 Liters per hour <5 Liters per hour
CO2 Control Range 2.9% - 9.9% 2.9% - 9.9%
O2 Control Range 2.0% - 20.0% 2.0% - 20.0%
Gas Input Pressure
 0.4 to 0.6 bar (5.80 - 8.70 PSI) 0.4 to 0.6 bar (5.80 - 8.70 PSI)
Operating Altitude Up to 2000 meters (6560 feet or 80kPa - 106kPa) Up to 2000 meters (6560 feet or 80kPa - 106kPa)
Shipping Weight 90 kg (198.4 lbs) (Including the pallet's weight) 120 kg (264.6 lbs) (Including the pallet's weight)
Shipping Dimensions 1080 x 705 x 670 mm (42.5 x 27.8 x 26.4") (device on the pallet) 1130 x 860 x 704 mm (44.5 x 33.9 x 27.7") (device on the pallet)

* Under normal condition (CO2 set point reached at 6.0%, all lids closed)
** Under normal condition (O2 set point reached at 5.0%, all lids closed)

Ordering Information

MIRI® Time-Lapse Incubator
Item Code Model Code Description
Unit
2070091 MRI-TL-MN-6C-8 MIRI® Time-Lapse Incubator, Mini, 6 Chambers, 230 V, 50/60 Hz
2070092 MRI-TL-MN-6C-9 MIRI® Time-Lapse Incubator, Mini, 6 Chambers, 115 V, 50/60 Hz
2070100 MRI-TL12C-8 MIRI® Time-Lapse Incubator, 12 Chambers, 230 V, 50/60 Hz
2070101 MRI-TL12C-9 MIRI® Time-Lapse Incubator, 12 Chambers, 115 V, 50/60 Hz
Accessories    
1320011 MRA-1007 VOC/HEPA filter (recommended to be changed every 3 months)
1320088 MRI-CC CultureCoin® for Time-Lapse of 14 embryos (25 pcs. per pack)
1320045 MRI-GA MIRI® GA CO2 /O2 & Temperature Validation Unit, 115V/ 230V
MIRI® Time-Lapse Viewer and Server
Item Code Model Code Description
2070042 MRI-VIEWER MIRI® Time-Lapse Viewer
1320095 MRI-SERVER MIRI® Time-Lapse Server

Literature & Resources